Dr. Zhang Receives Foundation for Anesthesia Education and Research (FAER) Training Grant
Zhang, M.D., Assistant Professor
We have been informed by our Chairman Dr. John Williams that Dr.
Li-Ming Zhang, former resident of anesthesiology and now Assistant
Professor is not only a newly Board Certified Anesthesiologist,
but also has had the distinction of earning a coveted Foundation
for Anesthesia Education and Research (FAER) training grant. The
title of Dr. Zhang's successful research application is "Chemical
biology of S-nitrosoalbumin in live pulmonary endothelial cells."
We congratulate Dr. Zhang on this most outstanding accomplishment.
Li-Ming Zhang, M.D., University of Pittsburgh School of Medicine,
Pittsburgh, Pennsylvania: “Chemical biology of S-nitrosoalbumin
in live pulmonary endothelial cells.” Mentor: Bruce R. Pitt,
Nitric oxide (NO) is a well known regulator of vascular homeostasis.
S-Nitrosoalbumin(SNO-Alb) is the most abundant physiological stable
circulating NO carrier in human plasma and also exerts vasodilatory
and antiaggregatory properties in vitro and in vivo. Recent studies
have showed that SNO-Alb produced a protective effect against
ischemia/reperfusion-induced microvascular constriction and edema
formation. In contrast to native albumin, the disposition of SNO-Alb
in pulmonary endothelium remains unclear.
In the current application, we will characterize the biological
effects of SNO-Alb within live intact rat pulmonary microvascular
endothelial cells via contemporary imaging approaches by detecting
(via real time confocal microscopy) its effect on fluorescence
resonance energy transfer (FRET) reporters of NO (cygnet-2, a
reporter of soluble guanylyl cyclase) or (FRET-metallothionein,
a reporter of S-nitrosation). Insight into the disposition of
SNO-Alb in pulmonary endothelial cells should provide new mechanistic
information on pulmonary vasomotor function and regulation of
water and solute exchange in health and disease.