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Structural Biology NMR Facility

Advanced facility housed inside the Biomedical Science Tower 3 (BST-3) building.

Bruker-BioSpin Avance 900 MHz NMR Spectrometer

  • 5mm HCN triple resonance cryoprobe yielding ultra high sensitivity
  • One of only a few magnets at this field strength in the world.

Bruker-BioSpin Avance 800 MHz NMR Spectrometer

  • 2.2k degree actively shielded 54mm bore super conducting magnets.
  • 34 gradient orthogonal shim systems.
  • 5mm HCN triple resonance cryoprobe yielding ultra high sensitivity.
  • 13C observe capability with increased sensitivity.
  • Solids and HRMAS capability (800 MHz).

Bruker-BioSpin Avance 700 MHz NMR Spectrometer

  • 5mm HCN triple resonance cryoprobe yielding ultra high sensitivity.

2 Bruker-BioSpin Avance 600 MHz NMR Spectrometers

  • 4.7k degree actively shielded 54mm bore magnets.
  • 34 gradient orthogonal shim systems.
  • 5mm HCN triple resonance cryoprobe yielding ultra high sensitivity.
  • 13C observe capability with increased sensitivity.

Bruker-BioSpin Avance 600WB MHz NMR Wide-Bore Spectrometer

  • 4.7k degree actively shielded 89 mm bore magnet.
  • 28 or 34 gradient orthogonal shim system operation.
  • 5mm HCN triple resonance high resolution probe.
  • Solid-State NMR (CPMAS, Static and HRMAS).
  • Imaging capabilities at various coil sizes.
  • Small animal imaging with physiologic monitoring.

All 6 magnets are controlled by Linux PC workstations, have variable temperature control, advanced digital receiving/digitizing capabilities, have an assortment of conventional high resolution probes, and are self-shielding (including the 900MHz)..

The Positron Emission Tomography (PET) Facilityhttp://www.pet.upmc.edu
The PET Facility supports a variety of research efforts in collaboration with faculty in the Departments of Psychiatry, Neurology, Radiology, Medicine, and Anesthesiology and the University of Pittsburgh Cancer Institute. It is noteworthy that researchers at this facility developed a prototype combined PET/CT scanner, and demonstrated this technology as the most powerful imaging tool available for localizing, evaluating and therapeutically monitoring head and neck cancer. The combined PET/CT scanner, known commercially as the Biograph, was FDA approved in 2001 as a diagnostic and therapeutic tool for cancer treatment.

  • Two scanning rooms, a control room, a data processing facility, a room for blood gas analysis and counting, as well as various patient waiting and preparation areas and offices.
  • The Radiochemistry Laboratory is part of the PET Facility Laboratory, and has rooms for both hot and cold work.
  • Uses a self shielded 11 MeV negative ion CTI RDS 112 cyclotron.

The Safar Center for Resuscitation Research (SCRR) http://www.safar.pitt.edu/ has animal laboratories, animal ICU facilities, and surgery rooms with appropriate equipment and set ups for:

  • Brain Trauma studies in rats, mice, and patients (in the hospitals across the street)
  • Hemorrhagic Shock (HS) studies in rats
  • Studies of HS and suspended animation in dogs
  • Outcome assessment, physiology, biochemistry, and molecular biology follow-up studies for the previously mentioned animal studies.
  • Access to UPMC South Side Hospital and UPMC Center for Sports Medicine.

General Clinical Research Center (GCRC)

  • Research encompasses the university’s six Schools of the Health Sciences, affiliated hospitals, specialized programs, and clinical facilities.
  • Facilities include inpatient beds, an outpatient suite, a metabolic kitchen, a DEXA scanner, and a sample processing laboratory.
  • Personnel include specialized research nurses, a bionutritionist, a research subject advocate, biostatisticians, information technologists, and support staff.
  • Satellite locations include Magee-Women’s Hospital Clinical Research Center, the Hillman Cancer Center, and the Western Psychiatric Institute and Clinical Neuroscience Research Center.
  • Laboratories supported by the GCRC include the Pharmacogenetics Core, laboratory, the PET Radiochemistry Laboratory, the Sleep and Circadian Rhythms Laboratory, and the Information Technology and Biostatistics Center.

On-campus support facilities provide the services listed below:

Biomedical Research Support Facility Center for Biotechnology and Bioengineering

  • Biosensor Facility – for real-time biomolecular interaction analysis via Biacore 3000 surface plasmon resonance system. (SPR measures changes in the refractive index at a surface.)
  • DNA Sequencing Core – for sequencing submitted DNA templates and primers.
  • Mass Spectrometry Core – accommodates both traditional electrospray ionization mass spectrometry and matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry.
  • Peptide Synthesis Core – provides comprehensive services for synthesis, purification, and characterization of synthetic peptides that are verified by mass spectrometry.  This facility has the capacity to produce and vial peptides under Good Laboratory Practice (GLP) conditions for use in human clinical trials.
  • Structural Biology Facility – houses an Aviv 62A DS Circular Dichroism (CD) Spectrometer and a companion Aviv UV-Vis Spectrophotometer. The instruments can allow for temperature control, total fluorescence, and automated titration.

Transgenic and Chimeric Mouse Facility

  • Provides a centralized service to produce transgenic and chimeric mice .
  • Services include DNA microinjection, mouse embryonic stem cell (ES cell) microinjection, embryo derivation, and cryopreservation.

Equipment includes:

  • Everything needed for protein expression and verification in bacteria, yeast, insect, or mammalian cells.  Supported Techniques: HPLC, affinity chromatography, RT PCR, PCR, lyophilization, ultra-centrifugation, gel electrophoresis, Western blotting, differential scanning calorimetry, isothermal calorimetry, light scattering, UV/Vis spectrometry, and so on.
  • Fluorescent and confocal microscopes with associated cameras and image processing software.
  • Histology tools including: microtomes for frozen or paraffin embedded sectioning, immunohistochemistry supplies, fluorescent labeling supplies, etc.
  • Tools for survival surgeries on rodents, such as: volatile anesthesia carts with vaporizers, rodent ventilators, polygraph machines to measure arterial pressure and such, ECG monitors, optical blood oxygen level monitoring clip for rodents, blood gas analyzer, drill and infusion pump for cranial injections, small animal monitoring stations for computerized radio-monitoring of anesthetized or awake rats (with intraperitoneal implanted transmitters),  etc…
  • Tools for survival surgeries on dogs, including: Grass and Schwann polygraphs, Novametric capnograph/O2 saturation monitors, external-internal defibrillators, anesthetic machine and vaporizer, electrocautery unit, Harvard ventilator, Baxter/Edwards Vigilance continuous cardiac output monitor, and so on...
  • MRI compatible animal monitoring equipment that allows for better image quality through physiological gating.  Cardiac and respiratory gating are possible, along with rectal temperature monitoring.  In magnet ventilation of the animal is also accommodated.
  • Radiolabeling equipment for PET studies including automatic liquid and gamma scintillation counters and a 90 MHz JEOL NMR spectrometer.